Abstract: Using microwave heating and electric furnace heating method,we comparatively studied the impact of two methodheating of Staphylococcus aureus on mice pathogenicity and bacilli inactivated. The results have showed that microwave treatmentsignificantly reduced bacterial virulence, pathogenicity weakened,and have proven that the non-thermal effects of microwavepathogenicity in mice and inactivated bacilli play an important role,which further validate the mechanism of microwave sterilization,but also provides a reference for the food industry to sterilization.