Abstract: To improve the optical frequency comb bandwidth and spectral flatness, an optical frequency comb generation scheme is designed based on polarization multiplexing technology and electric absorption modulator (EAM). By adjusting EAM parameters, a high flatness optical frequency comb is generated except the central carrier, and the peak power of central carrier is optimized to double the overall bandwidth of optical frequency comb using polarization multiplexing technology. The influence of relevant parameters on the flatness of optical frequency comb is discussed, and a frequency tunable and carrier reuse full-duplex radio over fiber (RoF) system based on this scheme is built by using Optisystem software. The results show that the power penalty of 10 km transmission is only 0. 42 dB compared with back-to-back transmission at Rbe = 10-9 in the downlink.